Wicketkeeper Alex Carey and paceman Jhye Richardson have become Australia's 223rd and 224th one-day international players, while Adam Zampa has been dropped for the second Gillette ODI against England at the Gabba.
Australia won the toss and elected to bat first as the hosts look to bounce back following their five-wicket defeat last Sunday.
Carey has come into the side in the absence of Tim Paine, who has been hit by a gastro bug and was ruled out of the match earlier today.
With paceman Josh Hazlewood also missing out due to illness and Pat Cummins rested, Western Australian Richardson has come into the side.
And in a surprise move, veteran batter Cameron White has been recalled at leg-spinner Zampa's expense, with the Victorian to play his first international match in nearly two years.
It means the make-up of the side is vastly different to that which played in the series opener; Richardson joins state teammate Andrew Tye, who made his debut last Sunday, and spearhead Mitchell Starc in a three-man frontline bowling attack, meaning allrounders Travis Head, Mitchell Marsh and Marcus Stoinis will likely have to bowl up to 20 overs between them.
Carey makes his international debut having struck a match-winning century for Adelaide Strikers in the KFC Big Bash League on Wednesday.
Only Hobart Hurricanes opener D'Arcy Short has scored more runs in BBL|07 than Carey, with his 368 runs coming at 61.33.
The 26-year-old also struck his maiden first-class century for South Australia last month, posting 139 against Queensland in their JLT Sheffield Shield match in Cairns.
Last summer, Carey broke the all-time Sheffield Shield dismissals record, effecting 59 dismissals to surpass Wade Seccombe's mark of 58 set in 2000-01.
He had also been touted as a bolter for the Magellan Ashes before Paine's eventual selection.
Richardson, a skiddy paceman from Western Australia, made his T20 International debut for Australia last summer against Sri Lanka. The right-armer was the player of the match in last season’s BBL final for champions Perth Scorchers.
Zampa's omission comes after he recorded his worst-ever ODI figures in the series-opener in Melbourne.
White, who could pitch in with his part-time leg-spin in the absence of a specialist slow bowler, last played for Australia in January 2015.
Hazlewood and Paine are the latest players to be struck down by illness in recent weeks, joining David Warner, Steve Smith and Patrick Cummins as well as England captain Joe Root and batsman James Vince.
AUSTRALIA: David Warner, Aaron Finch, Steve Smith (c), Travis Head, Cameron White, Mitchell Marsh, Marcus Stoinis, Alex Carey (wk), Mitchell Starc, Jhye Richardson, Andrew Tye.
ENGLAND: Jason Roy, Jonny Bairstow, Alex Hales, Joe Root, Eoin Morgan (c), Jos Buttler (wk), Moeen Ali, Chris Woakes, Adil Rashid, Liam Plunkett, Mark Wood
2017-18 International Fixtures
Gillette ODI Series v England
Australia ODI squad: Steve Smith (c), David Warner (vc), Pat Cummins, Aaron Finch, Josh Hazlewood, Travis Head, Mitchell Marsh, Tim Paine, Jhye Richardson, Mitchell Starc, Marcus Stoinis, Andrew Tye, Cameron White, Adam Zampa.
England ODI squad: Eoin Morgan (c), Moeen Ali, Jonny Bairstow, Jake Ball, Sam Billings, Jos Buttler, Tom Curran, Alex Hales, Dawid Malan, Liam Plunkett, Adil Rashid, Joe Root, Jason Roy, David Willey, Chris Woakes, Mark Wood.
First ODI England won by five wickets at the MCG.
Second ODI Gabba, January 19. Tickets
Third ODI SCG, January 21. Tickets
Fourth ODI Adelaide Oval, January 26. Tickets
Fifth ODI Perth Stadium, January 28. Tickets
Prime Minister's XI
PM's XI v England Manuka Oval, February 2. Tickets
Gillette T20 trans-Tasman Tri-Series
England T20 squad: Eoin Morgan (c), Sam Billings, Jos Buttler, Tom Curran, Liam Dawson, Alex Hales, Chris Jordan, Dawid Malan, Liam Plunkett, Adil Rashid, Joe Root, Jason Roy, Ben Stokes, James Vince, David Willey, Mark Wood.
First T20I Australia v NZ, SCG, February 3. Tickets
Second T20I Australia v England, Blundstone Arena, February 7. Tickets
Third T20I Australia v England, MCG, February 10. Tickets
Fourth T20I NZ v England, Wellington, February 14
Fifth T20I NZ v Australia, Eden Park, February 16
Sixth T20I NZ v England, Seddon Park, February 18
Final TBC, Eden Park, February 21